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產(chǎn)品中心Products 當(dāng)前位置:首頁 > 產(chǎn)品中心 > 分子生物學(xué) > 原子力顯微鏡 > 超高速視頻級原子力顯微鏡—HS-AFM

超高速視頻級原子力顯微鏡—HS-AFM

超高速視頻級原子力顯微鏡—HS-AFM

更新時間:2026-05-11

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HS-AFM超高速視頻級原子力顯微鏡—HS-AFM的詳細資料:

簡介
超高速視頻級原子力顯微鏡(HS-AFM)是一種突破傳統(tǒng)AFM掃描成像速度限制的高分辨率顯微鏡,可在液態(tài)環(huán)境下實現(xiàn)超快速動態(tài)成像,特別適合生物大分子動態(tài)觀測。該設(shè)備由日本RIBM公司生產(chǎn),具有20幀/秒的掃描速度,廣泛應(yīng)用于生物物理、生物化學(xué)等領(lǐng)域,已幫助科學(xué)家在期刊上發(fā)表了300多篇論文。HS-AFM能夠?qū)崟r觀察單個分子級別的動態(tài)變化,無需特殊固定處理,對生物樣品友好,適用于長時間觀測。

其它參數(shù)

掃描速度:20 frame/s
XY軸分辨率:2nm
Z軸分辨率:0.5nm

超高速視頻級原子力顯微鏡—HS-AFM

 

原子力顯微鏡(Atomic Force Microscope,AFM),一種可用來研究包括絕緣體在內(nèi)的固體材料表面結(jié)構(gòu)的分析儀器。它通過檢測待測樣品表面和一個微型力敏感元件之間的極微弱的原子間相互作用力來研究物質(zhì)的表面結(jié)構(gòu)及性質(zhì)。將一對微弱力敏感的微懸臂一端固定,另一端的微小針尖接近樣品,這時它將與其相互作用,作用力將使得微懸臂發(fā)生形變或運動狀態(tài)發(fā)生變化。掃描樣品時,利用傳感器檢測這些變化,就可獲得作用力分布信息,從而以納米級分辨率獲得表面形貌結(jié)構(gòu)信息及表面粗糙度信息。原子力顯微鏡可以測量材料物理性質(zhì)、力學(xué)性能、磁學(xué)性能、熱學(xué)性能、電學(xué)性能等方面的一些特征信息,但在掃描成像速度上一直存在局限性,太慢的掃描速度導(dǎo)致原子力顯微鏡無法捕捉到分子間的相互作用過程和一些快速的分子動態(tài)變化。

 

超高速視頻級原子力顯微鏡(High-Speed Atomic Force Microscope,HS-AFM)由日本 Kanazawa 大學(xué) Prof. Ando 教授團隊研發(fā),日本RIBM公司(生體分子計測研究所株式會社,Research Institute of Biomolecule Metrology Co., Ltd)商業(yè)化的產(chǎn)品,可以達到視頻級成像的商業(yè)化原子力顯微鏡。HS-AFM突破了傳統(tǒng)原子力顯微鏡“掃描成像速慢"的限制,能夠在液體環(huán)境下超快速動態(tài)成像,分辨率為納米水平。樣品無需特殊固定,不影響生物分子的活性,尤其適用于生物大分子互作動態(tài)觀測。超高速視頻級原子力顯微鏡HS-AFM主要有兩種型號,SS-NEX樣品掃描(Sample-Scanning HS-AFM)以及PS-NEX探針掃描(Probe-Scanning HS-AFM)。推出至今,已有150多位用戶,發(fā)表 SCI 文章 300 余篇,包括Science, Nature, Cell 等雜志。

 

相較于目前市場上的原子力顯微鏡成像設(shè)備,HS-AFM突破了 “掃描成像速慢"的限制,掃描速度高可達 20 frame/s,并且有 4 種掃描臺可供選擇。樣品無需特殊固定染色,不影響生物分子的活性,尤其適用于生物大分子互作動態(tài)觀測。液體環(huán)境下直接檢測,超快速動態(tài)成像,分辨率為納米水平。探針小,適用于生物樣品;懸臂探針共振頻率高,彈簧系數(shù)小,避免了對生物樣品等的損傷。懸臂探針可自動漂移校準(zhǔn),適用于長時間觀測。采用動態(tài)PID控制,高速掃描時仍可獲得清晰的圖像。XY軸分辨率2nm;Z軸分辨率0.5nm。

HS-AFM不僅擁有超高掃描速率與原子級別分辨率,而且具有操作的簡易性,使得對單分子動態(tài)過程的捕捉變得十分方便,為科研工作者研所和理解生物物理、生物化學(xué)、分子生物學(xué)、病毒學(xué)以及生物醫(yī)學(xué)等領(lǐng)域的單分子動態(tài)過程提供了一款的工具。

全新的HS-AFM采用了新的高頻微懸臂架構(gòu),更低噪音、更高穩(wěn)定性的2控制器,高速掃描器,緩沖防震設(shè)計,主動阻尼,動態(tài)PID,驅(qū)動算法優(yōu)化,多種前沿技術(shù),可以實現(xiàn)在超高速下獲取高分辨的生物樣品信息。新系統(tǒng)整合了基于工作流程的操作軟件,直觀的用戶界面與流程化、自動化的設(shè)置使得研究人員可以專注于實驗設(shè)計,不需要復(fù)雜的操作和條件設(shè)置,快速獲取數(shù)據(jù),加速研究的產(chǎn)出。

 


日本RIBM公司的超高速視頻級原子力顯微鏡HS-AFM的創(chuàng)新點:

★   高頻微懸臂

彈性系數(shù):   0.1 N/m   

曲率半徑:   <10 nm 

共振頻率:   400-600kHz in liquid

★   高速掃描臺

20 frames/s.   (Standard scanner)

★   緩沖防震+主動阻尼+動態(tài)PID+算法優(yōu)化

緩沖防震

主動阻尼

動態(tài)PID控制:可自動改變反饋增益,保證了HS-AFM在高速掃描條件下仍可獲得清晰的圖像

探針自動漂移校準(zhǔn),適用于長時間樣品觀測

 

     

 

日本RIBM公司的超高速視頻級原子力顯微鏡HS-AFM的的應(yīng)用領(lǐng)域:

 

從單分子到單細胞,都可直接觀測

 

 

1、肌動蛋白

 

2、CRISPR-Cas9

 

 

3、膜聯(lián)蛋白

 

 

4、IgG

 

 

5、活細胞

 

 

6、細菌視紫紅質(zhì)

 

 

7、DNA納米結(jié)構(gòu)

 

 

8、仿生聚合物

 

 

日本RIBM公司的超高速視頻級原子力顯微鏡HS-AFM的視頻案例

 

1:IgG

在溶液中觀察到抗體(IgG)。

IgG呈"Y"形,兩個Fab區(qū)區(qū)分清晰。

由于錨定能力較弱,IgG保持其抗原結(jié)合能力。

 

 

 

2:Plasmid DNA

傳統(tǒng)AFM在沒有強錨定的情況下,DNA分子圖像出現(xiàn)擺動。

然而,強錨定可能會削弱真實的結(jié)構(gòu)和行為。

HS-AFM能清晰顯示質(zhì)粒的結(jié)構(gòu)和運動,無強錨定。

 

 

 

3:DNA內(nèi)切酶的消化:DNase I

DNA酶I是一種隨機消化DNA的核酸內(nèi)切酶。視頻中的箭頭表示DNase I消化DNA的部分。

請參考從DNA末端消化的核酸外切酶Bal31的視頻。

 

 

 

4:DNA外切酶消化:Bal31

Bal31是一種從DNA鏈末端消化DNA的核酸外切酶。

視頻顯示Bal31的活性沿著DNA移動,并逐漸從DNA鏈的末端消化。

最后,DNA分子被消化,但環(huán)狀DNA未被消化。高光點是Bal31分子,它們與DNA的不同位置結(jié)合。
 

 

 

5:DNA聚合酶的DNA延伸:Phi29

雙鏈DNA(黃色)隨著時間的推移而拉長。單鏈λDNA作為模具固定在基板上。

由于從隨機六聚體引物(Red)結(jié)合到λDNA模體,phi29聚合酶(Black)以dNTP為底物合成互補DNA。
 

 

 

6:鏈親和素2D晶體中的點缺陷

成功地觀察到點缺陷在晶體中的擴散。

從圖像上看,兩個單空位缺陷的軌跡跟蹤相對于晶格的兩個軸是明顯的各向異性的。

 

 

 

 

日本RIBM公司的超高速視頻級原子力顯微鏡HS-AFM的文獻列表

 

Title

Journal

Biophysical reviews top five: atomic force microscopy in biophysics

Biophysical Reviews

Reconstruction of Three-Dimensional Conformations of Bacterial
ClpB from High-Speed Atomic-Force-Microscopy Images

Frontiers in Molecular
Biosciences

A facile combinatorial approach to construct a ratiometric fluorescent
sensor: application for the real-time sensing of cellular pH changes

Chemical Science

DNA Nanotechnology to Disclose Molecular Events at the Nanoscale
and Mesoscale Levels

Springer Nature

Quantitative description of a contractile macromolecular machine

Science Advances

Dynamic Assembly/Disassembly of Staphylococcus aureus FtsZ
Visualized by High-Speed Atomic Force Microscopy

International Journal of
Molecular Sciences 2021, Vol. 22, Page 1697

Localization atomic force microscopy

Nature 2021 594:7863

Movements of mycoplasma mobile gliding machinery detected by
high-speed atomic force microscopy

mBio

An ultra-wide scanner for large-area high-speed atomic force
microscopy with megapixel resolution

Scientific Reports 2021 11:1

A molecularly engineered, broad-spectrum anti-coronavirus lectin
inhibits SARS-CoV-2 and MERS-CoV infection in vivo

Research Square

Influenza virus ribonucleoprotein complex formation occurs in the
nucleolus

bioRxiv

Tardigrade Secretory-Abundant Heat-Soluble Protein Has a Flexible
β-Barrel Structure in Solution and Keeps This Structure in
Dehydration

Journal of Physical Chemistry B

Ultrastructure of influenza virus ribonucleoprotein complexes during
viral RNA synthesis

Communications Biology on

A facile combinatorial approach to construct a ratiometric fluorescent
sensor: application for the real-time sensing of cellular pH changes

Chemical Science

Deformation of microtubules regulates translocation dynamics of
kinesin

Science Advances

Unraveling the host-selective toxic interaction of cassiicolin with lipid
membranes and its cytotoxicity

bioRxiv

Nanostructure and thermoresponsiveness of poly( N -isopropyl
methacrylamide)-based hydrogel microspheres prepared via aqueous free radical precipitation polymerization

RSC Advances

JRAB/MICAL-L2 undergoes liquid–liquid phase separation to form
tubular recycling endosomes

Communications Biology 2021
4:1

Correlation of membrane protein conformational and functional
dynamics

Nature Communications 2021
12:1

Non-close-packed arrangement of soft elastomer microspheres on
solid substrates

RSC Advances

Folding RNA–Protein Complex into Designed Nanostructures

Methods in Molecular Biology

A glutamine sensor that directly activates TORC1

Communications Biology 2021
4:1

Architecture of zero-latency ultrafast amplitude detector for high-
speed atomic force microscopy

Applied Physics Letters

Correlative AFM and fluorescence imaging demonstrate nanoscale
membrane remodeling and ring-like and tubular structure formation by septins

Nanoscale

Desiccation-induced fibrous condensation of CAHS protein from an
anhydrobiotic tardigrade

bioRxiv

Construction of ferritin hydrogels utilizing subunit–subunit
interactions

PLOS ONE

Monomeric α-synuclein (αS) inhibits amyloidogenesis of human
prion protein (hPrP) by forming a stable αS-hPrP hetero-dimer.

Prion

Influence of protein adsorption on aggregation in prefilled syringes

Journal of Pharmaceutical
Sciences

Dynamic mechanisms of CRISPR interference by Escherichia coli
CRISPR-Cas3

bioRxiv

An RNA Triangle with Six Ribozyme Units Can Promote a Trans-
Splicing Reaction through Trimerization of Unit Ribozyme Dimers

Applied Sciences

Faster high-speed atomic force microscopy for imaging of
biomolecular processes

Rev. Sci. Instrum

Title: Identification of lectin receptors for conserved SARS-CoV-2
glycosylation sites

bioRxiv

Structural and dynamics analysis of intrinsically disordered proteins
by high-speed atomic force microscopy

Nature Nanotechnology

Millisecond Conformational Dynamics of Skeletal Myosin II Power
Stroke Studied by High-Speed Atomic Force Microscopy

ACS Nano

High-Speed Atomic Force Microscopy Reveals Spatiotemporal
Dynamics of Histone Protein H2A Involution by DNA Inchworming

The Journal of Physical
Chemistry Letters

Nanostructures, Thermoresponsiveness, and Assembly Mechanism
of Hydrogel Microspheres during Aqueous Free-Radical Precipitation Polymerization

Langmuir

Chained structure of dimeric F 1-like ATPase in Mycoplasma mobile
gliding machinery 4

bioRxiv

Lipid Membrane Interaction of Peptide/DNA Complexes Designed for
Gene Delivery

Langmuir

High-Speed Atomic Force Microscopy to Study Myosin Motility

Advances in Experimental
Medicine and Biology

Single-molecule level dynamic observation of disassembly of the
apo-ferritin cage in solution

Physical Chemistry Chemical
Physics

Atomic Force Microscopy of Biomembranes : A Tool for Studying the
Dynamic Behavior of Membrane Proteins

New Techniques for Studying
Biomembranes

Adenosine leakage from perforin-burst extracellular vesicles inhibits
perforin secretion by cytotoxic T-lymphocytes

PLOS ONE

High-Speed Atomic Force Microscopy Reveals the Structural
Dynamics of the Amyloid-β and Amylin Aggregation Pathways

International Journal of
Molecular Sciences 2020, Vol. 21, Page 4287

Molecular mechanism of the recognition of bacterially cleaved
immunoglobulin by the immune regulatory receptor LILRA2

Journal of Biological Chemistry

Biological physics by high-speed atomic force microscopy

Philosophical Transactions of
the Royal Society A: Mathematical, Physical and Engineering Sciences

Viral RNA recognition by LGP2 and MDA5, and activation of signaling
through step-by-step conformational changes

Nucleic Acids Research

Key Nucleation Stages and Associated Molecular Determinants and
Processes in pH-Induced Formation of Amyloid Beta Oligomers as Revealed by High-Speed AFM

bioRxiv

Novel Babesia bovis exported proteins that modify properties of
infected red blood cells

PLoS Pathogens

DNA origami demonstrate the unique stimulatory power of single
pMHCs as T-cell antigens

Proceedings of the National
Academy of Sciences

Structural insights into the mechanism of rhodopsin
phosphodiesterase

Nature Communications

Structural insights into the mechanism of rhodopsin
phosphodiesterase

Nature Communications

High-Speed AFM Reveals Molecular Dynamics of Human Influenza A
Hemagglutinin and Its Interaction with Exosomes

Nano Letters

DNA Ring Motif with Flexible Joints

Micromachines

Nanopores: a versatile tool to study protein dynamics

Essays in Biochemistry

Geometrical Characterization of Glass Nanopipettes with Sub-10 nm
Pore Diameter by Transmission Electron Microscopy

Analytical Chemistry

Carbon nanotube porin diffusion in mixed composition supported
lipid bilayers

Scientific Reports

One-Step Calibration of AFM in Liquid

Frontiers in Physics

Nanoscale interaction of RecG with mobile fork DNA

Nanoscale Advances

Convergent evolution of processivity in bacterial and fungal
cellulases

Proceedings of the National
Academy of Sciences

Convergent evolution of processivity in bacterial and fungal
cellulases

Proceedings of the National
Academy of Sciences of the United States of America

Interaction of the motor protein SecA and the bacterial protein
translocation channel SecYEG in the absence of ATP

Nanoscale Advances

Nanoreporter of an Enzymatic Suicide Inactivation Pathway

Nano Letters

High-speed atomic force microscopy highlights new molecular
mechanism of daptomycin action

Nature Communications

Direct visualization of the conformational change of FUS/TLS upon
binding to promoter-associated non-coding RNA

Chemical Communications

Thermoresponsive Micellar Assembly Constructed from a Hexameric
Hemoprotein Modified with Poly(N-isopropylacrylamide) toward an Artificial Light-Harvesting System

Journal of the American
Chemical Society

Schizorhodopsins: A family of rhodopsins from Asgard archaea that
function as light-driven inward H + pumps

Science Advances

Two-State Exchange Dynamics in Membrane-Embedded
Oligosaccharyltransferase Observed in Real-Time by High-Speed AFM

Journal of Molecular Biology

Thermoresponsive structural changes of single poly(N-isopropyl
acrylamide) hydrogel microspheres under densely packed conditions on a solid substrate

Polymer Journal

High-Speed Atomic Force Microscopy Reveals Factors Affecting the
Processivity of Chitinases during Interfacial Enzymatic Hydrolysis of Crystalline Chitin

ACS Catalysis

Rad50 zinc hook functions as a constitutive dimerization module
interchangeable with SMC hinge

Nature Communications

Assembly mechanism of a supramolecular MS-ring complex to
initiate bacterial flagellar biogenesis in vibrio species

Journal of Bacteriology

Structural Dynamics of a Protein Domain Relevant to the Water-
Oxidizing Complex in Photosystem II as Visualized by High-Speed Atomic Force Microscopy

Journal of Physical Chemistry B

Recent advances in bioimaging with high-speed atomic force
microscopy

Biophysical Reviews

Dynamic behavior of an artificial protein needle contacting a
membrane observed by high-speed atomic force microscopy

Nanoscale

Enhanced enzymatic activity exerted by a packed assembly of a
single type of enzyme

Chemical Science

High Speed AFM and NanoInfrared Spectroscopy Investigation of A
β1–42 Peptide Variants and Their Interaction With
POPC/SM/Chol/GM1 Model Membranes

Frontiers in Molecular
Biosciences

The hierarchical assembly of septins revealed by high-speed AFM

Nature Communications

Millisecond dynamics of an unlabeled amino acid transporter

Nature Communications

Atg9 is a lipid scramblase that mediates autophagosomal membrane
expansion

Nature Structural & Molecular
Biology

A Simplified Cluster Analysis of Electron Track Structure for
Estimating Complex DNA Damage Yields

International Journal of
Molecular Sciences

Supramolecular tholos-like architecture constituted by archaeal
proteins without functional annotation

Scientific Reports

Studies on the impellers generating force in muscle

Biophysical Reviews

Diversity of physical properties of bacterial extracellular membrane
vesicles revealed through atomic force microscopy phase imaging

Nanoscale

High-Speed AFM Reveals Molecular Dynamics of Human Influenza A
Hemagglutinin and Its Interaction with Exosomes

Nano Letters

DNA density-dependent uptake of DNA origami-based two-or three-
dimensional nanostructures by immune cells

Nanoscale

Spatiotemporally tracking of nano-biofilaments inside the nuclear
pore complex core

Biomaterials

High-speed atomic force microscopy directly visualizes
conformational dynamics of the HIV Vif protein in complex with three host proteins

Journal of Biological Chemistry

Self- and Cross-Seeding on α-Synuclein Fibril Growth Kinetics and
Structure Observed by High-Speed Atomic Force Microscopy

ACS Nano

Liquidity Is a Critical Determinant for Selective Autophagy of Protein
Condensates

Molecular Cell

Capturing transient antibody conformations with DNA origami
epitopes

Nature Communications

Biophysics in Kanazawa University

Biophysical Reviews

Dynamics of oligomer and amyloid fibril formation by yeast prion
Sup35 observed by high-speed atomic force microscopy

Proceedings of the National
Academy of Sciences

Annexin-V stabilizes membrane defects by inducing lipid phase
transition

Nature Communications

Structure and mechanism of bactericidal mammalian perforin-2, an
ancient agent of innate immunity

Science Advances

Atomic Force Microscopy Visualizes Mobility of Photosynthetic
Proteins in Grana Thylakoid Membranes

Biophysical Journal

Construction of a Hexameric Hemoprotein Sheet and Direct
Observation of Dynamic Processes of Its Formation

Chemistry Letters

Single-molecule imaging analysis reveals the mechanism of a high-
catalytic-activity mutant of chitinase A from Serratia marcescens

Journal of Biological Chemistry

Direct observation and analysis of TET-mediated oxidation
processes in a DNA origami nanochip

Nucleic Acids Research

On-membrane dynamic interplay between anti-GM1 IgG antibodies
and complement component C1q

International Journal of
Molecular Sciences

Zwitterionic Polypeptides: Chemoenzymatic Synthesis and Loosening
Function for Cellulose Crystals

Biomacromolecules

 

 


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